Kooiman's early work in TV broadcast journalism began with her working as a sideline reporter for Fox Sports during her college years at UNC Wilmington in 2004. After graduation, she continued her work as a reporter, anchor and video Journalist for WWAY in Wilmington, North Carolina in 2005. In January 2007 Kooiman moved to Toledo, Ohio, and began working for the local NBC affiliate, WNWO, as morning anchor and reporter. While there, she served as franchise reporter for a partnership with U.S. Marshals that helped catch over 100 Fugitives. In May 2008, Kooiman moved back to Charlotte, North Carolina, to work as an anchor/reporter/host for Fox News Rising, a four-hour news and entertainment morning show on the then-FOX affiliate WCCB (as of 2013, WCCB became a CW-affiliated network). While at WCCB-TV, she reported on local news stories, entertainment, weather and traffic.

Kooiman served as the philanthropy chair of her sorority, Alpha Delta Pi while at University of North Carolina at Wilmington. Beginning in August 2008, Kooiman was a fitness instructor in Charlotte. She is AFAA and Turbo Kick Certified. She taught up to ten classes each week. She also served as a fitness instructor to kids in an inner-city after-school program.

In December 2011, Kooiman became a Fox News Channel New York-based correspondent covering health and fitness as well as general assignment reporting. She was also host on the weekend edition of FNC's FOX & Friends. Kooiman covered terror attacks in San Bernardino, California, and Orlando, Florida. She was also out in the elements during major weather events like Super Storm Sandy, tornadoes in Moore, Oklahoma, and the 2016 U.S. blizzard. Kooiman covered the Fox debates for the GOP primary in Detroit. She has also hosted the All American New Year for FNC. Her franchise, "Roadtrip with Anna Kooiman" takes viewers around the United States, checking off bucket list items. She also hosted Fox News Extra segments.

Kooiman grew up in Charlotte, North Carolina, where her parents still live and own businesses – Southeast Oasis Pool and the Peppermint Forest Christmas Shop. Of Dutch ancestry, she went to Myers Park High School (2002 graduate), where she played softball and also played with the SouthPark Youth Association.

On February 8, 2015, Kooiman announced her engagement on her Facebook page. She became engaged to her boyfriend Tim Stuckey while on a personal vacation to Australia; her fiance proposing to her while both were watching the sunrise over Sydney Harbour. The couple was married on July 31, 2015, at Manhattan's Central Park Loeb Boathouse. Anna's husband appeared in 2016 on FOX & Friends for a cooking segment, which received social media attention for using the signature Aussie condiment, Vegemite. Kooiman is known on social media for her international travel and adventures.

In April 2016, Kooiman was crowned Festival Queen for the North Carolina Azalea Festival.