Ashley Tesoro grew up singing in church and performing in the theater company Christian Youth Theater. She and her husband Anthony are both Christian ministers, and run the Tesoro Ministry Foundation, a non-profit charity-ministry foundation founded by Anthony in 2000. She is the co-writer of Life Is For Living; a Christian ministry and lifestyle blog, and the co-host of Life Is For Living TV a Christian ministry and lifestyle web-series.

On September 30, 2001, Ashley Cafagna married Anthony Tesoro, a Christian minister. Anthony formerly worked on Wall Street for Paine Webber and then Jones Institutional Trading Services before entering the non-profit sector and founding ministry/charity organization the Tesoro Ministry Foundation, Inc. in 2000. In 2005 Ashley and her husband Anthony founded Tesoro Entertainment, a production company; and in 2007 they launched Tesoro Entertainment's music label division, Tesoro Records; with the Christian music sub-label Sacred Music launching in 2012. Their daughter, Gabriella Lyn, was born on November 18, 2013; and their son Anthony John, was born December 24, 2015.

While starring on The Bold and the Beautiful, executive-producer Bradley Bell incorporated singing into her character of Kimberly Fairchild. She recorded several original songs and music videos for the show. One of these songs was premiered on KIIS-FM's Valentine Show. In 2007 Ashley Tesoro made her country music debut with a self-titled EP on Tesoro Records. The album was produced by Ron Aniello, mixed by Mike Shipley, and mastered by Joe Gastwirt. Larry Eagle, Drummer and percussionist on Bruce Springsteen's We Shall Overcome: The Seeger Sessions, played drums on two of the EP's tracks. In 2010 she released her second EP on Tesoro Records titled Oh You Angel. This country gospel album was produced by Ron Aniello, mixed by Clif Norrell, and mastered by Joe Gastwirt. In August 2010 Ashley Tesoro opened for Jackson Browne at a private event in Santa Barbara, California. Supported by the Abiders she performed the song "Heaven Let Me Know" from her EP Oh You Angel. In 2012 she released her third EP on Tesoro Records titled Simply Worship. This A cappella and acoustic gospel album was produced by Ron Aniello and mixed and mastered by Alec Dixon.

As a television Actress Ashley Tesoro has appeared in NBC’s Saved by the Bell: The New Class (as Liz Miller) for three seasons and CBS’s The Bold and the Beautiful (as Kimberly Fairchild) for three years. She has had other roles in 7th Heaven, Valley of the Dolls, Hey Arnold!, CBS Morning News, and has been a guest host for E!. She has presented the Daytime Emmy Awards, the Soap Opera Digest Awards, and was awarded "Best Newcomer on a Daytime Television Show" in Australia. She was also named one of the "Fifty most Beautiful People" on television by Soap Opera Digest and Soap Opera Weekly. She has also starred in eight films during her career, including; The Skulls II, Lord of Illusions playing Famke Janssen's younger self, and Mr. Murder with Stephen Baldwin, Thomas Haden Church, and James Coburn.