Born in St. Louis, Missouri, Berry was the fourth child in a family of six. He grew up in the north St. Louis neighborhood known as the Ville, an area where many middle-class people lived. His father, Henry william Berry (1895–1987), was a contractor and deacon of a nearby Baptist church; his mother, Martha Bell (Banks) (1894–1980), was a certified public school principal. Berry's upbringing allowed him to pursue his interest in music from an early age. He gave his first public performance in 1941 while still a student at Sumner High School; he was still a student there in 1944, when he was arrested for armed robbery after robbing three shops in Kansas City, Missouri, and then stealing a car at gunpoint with some friends. Berry's account in his autobiography is that his car broke down and he flagged down a passing car and stole it at gunpoint with a nonfunctional pistol. He was convicted and sent to the Intermediate Reformatory for Young Men at Algoa, near Jefferson City, Missouri, where he formed a singing quartet and did some boxing. The singing group became competent enough that the authorities allowed it to perform outside the detention facility. Berry was released from the reformatory on his 21st birthday in 1947.

Born into a middle-class African-American family in St. Louis, Missouri, Berry had an interest in music from an early age and gave his first public performance at Sumner High School. While still a high school student he was convicted of armed robbery and was sent to a reformatory, where he was held from 1944 to 1947. After his release, Berry settled into married life and worked at an automobile assembly plant. By early 1953, influenced by the guitar riffs and showmanship techniques of the blues musician T-Bone Walker, Berry began performing with the Johnnie Johnson Trio. His break came when he traveled to Chicago in May 1955 and met Muddy Waters, who suggested he contact Leonard Chess, of Chess Records. With Chess, he recorded "Maybellene"—Berry's adaptation of the country song "Ida Red"—which sold over a million copies, reaching number one on Billboard magazine's rhythm and blues chart. By the end of the 1950s, Berry was an established star, with several hit records and film appearances and a lucrative touring career. He had also established his own St. Louis nightclub, Berry's Club Bandstand. But in January 1962, he was sentenced to three years in prison for offenses under the Mann Act—he had transported a 14-year-old girl across state lines. After his release in 1963, Berry had several more hits, including "No Particular Place to Go", "You Never Can Tell", and "Nadine". But these did not achieve the same success, or lasting impact, of his 1950s songs, and by the 1970s he was more in demand as a nostalgic performer, playing his past hits with local backup bands of variable quality. His insistence on being paid in cash led in 1979 to a four-month jail sentence and community Service, for tax evasion.

On October 28, 1948, Berry married Themetta "Toddy" Suggs, who gave birth to Darlin Ingrid Berry on October 3, 1950. Berry supported his family by taking various jobs in St. Louis, working briefly as a factory worker at two automobile assembly plants and as a janitor in the apartment building where he and his wife lived. Afterwards he trained as a beautician at the Poro College of Cosmetology, founded by Annie Turnbo Malone. He was doing well enough by 1950 to buy a "small three room brick cottage with a bath" on Whittier Street, which is now listed as the Chuck Berry House on the National Register of Historic Places.

When asked what caused the explosion of the popularity of rock 'n roll that took place in the 1950s, with him and a handful of others, mainly him, Berry said, "Well, actually they begin to Listen to it, you see, because certain stations played certain music. The music that we, the blacks, played, the cultures were so far apart, we would have to have a play station in order to play it. The cultures begin to come together, and you begin to see one another's vein of life, then the music came together."

By early 1953 Berry was performing with Johnnie Johnson's trio, starting a long-time collaboration with the Pianist. The band played mostly blues and ballads, but the most popular music among whites in the area was country. Berry wrote, "Curiosity provoked me to lay a lot of our country stuff on our predominantly black audience and some of our black audience began whispering 'who is that black hillbilly at the Cosmo?' After they laughed at me a few times they began requesting the hillbilly stuff and enjoyed dancing to it."

A pioneer of rock and roll, Berry was a significant influence on the development of both the music and the attitude associated with the rock music lifestyle. With songs such as "Maybellene" (1955), "Roll Over Beethoven" (1956), "Rock and Roll Music" (1957) and "Johnny B. Goode" (1958), Berry refined and developed rhythm and blues into the major elements that made rock and roll distinctive, with lyrics successfully aimed to appeal to the early teenage market by using graphic and humorous descriptions of teen dances, fast cars, high school life, and consumer culture, and utilizing guitar solos and showmanship that would be a major influence on subsequent rock music. Thus Berry, the Songwriter, invented rock as "a music of teenage wishes fulfilled and good times (even with cops in pursuit)." Berry contributed three things to rock music: an irresistible swagger, a focus on the guitar riff as the primary melodic element and an emphasis on songwriting as storytelling. His records are a rich storehouse of the essential lyrical, showmanship and musical components of rock and roll. In addition to the Beatles and the Rolling Stones, a large number of significant popular-music performers have recorded Berry's songs. Though not technically accomplished, his guitar style is distinctive—he incorporated electronic effects to mimic the sound of bottleneck blues guitarists and drew on the influence of guitar players such as Carl Hogan, and T-Bone Walker to produce a clear and exciting sound that many later guitarists would acknowledge as an influence in their own style. Berry's showmanship has been influential on other rock guitarists, particularly his one-legged hop routine, and the "duck walk", which he first used as a child when he walked "stooping with full-bended knees, but with my back and head vertical" under a table to retrieve a ball and his family found it entertaining; he used it when "performing in New York for the first time and some Journalist branded it the duck walk."

At the end of June 1956, his song "Roll Over Beethoven" reached number 29 on the Billboard's Top 100 chart, and Berry toured as one of the "Top Acts of '56". He and Carl Perkins became friends. Perkins said that "I knew when I first heard Chuck that he'd been affected by country music. I respected his writing; his records were very, very great." As they toured, Perkins discovered that Berry not only liked country music but also knew about as many songs as he did. Jimmie Rodgers was one of his favorites. "Chuck knew every Blue Yodel and most of Bill Monroe's songs as well", Perkins remembered. "He told me about how he was raised very poor, very tough. He had a hard life. He was a good guy. I really liked him."

In late 1957, Berry took part in Alan Freed's "Biggest Show of Stars for 1957", touring the United States with the Everly Brothers, Buddy Holly, and others. He was a guest on ABC's Guy Mitchell Show, singing his hit song "Rock 'n' Roll Music". The hits continued from 1957 to 1959, with Berry scoring over a dozen chart singles during this period, including the US Top 10 hits "School Days", "Rock and Roll Music", "Sweet Little Sixteen", and "Johnny B. Goode". He appeared in two early rock-and-roll movies: Rock Rock Rock (1956), in which he sang "You Can't Catch Me", and Go, Johnny, Go! (1959), in which he had a speaking role as himself and performed "Johnny B. Goode", "Memphis, Tennessee", and "Little Queenie". His performance of "Sweet Little Sixteen" at the Newport Jazz Festival in 1958 was captured in the motion picture Jazz on a Summer's Day.

When Berry was released from prison in 1963 his return to recording and performing was made easier because British invasion bands—notably the Beatles and the Rolling Stones—had sustained interest in his music by releasing cover versions of his songs, and other bands had reworked some of them, such as the Beach Boys' 1963 hit "Surfin' U.S.A.", which used the melody of Berry's "Sweet Little Sixteen". In 1964 and 1965 Berry released eight singles, including three that were commercially successful, reaching the top 20 of the Billboard 100: "No Particular Place to Go" (a humorous reworking of "School Days", concerning the introduction of seat belts in cars), "You Never Can Tell", and the rocking "Nadine". Between 1966 and 1969 Berry released five albums for Mercury Records, including his first live album, Live at Fillmore Auditorium, in which he was backed by the Steve Miller Band.

While this was not a successful period for studio work, Berry was still a top concert draw. In May 1964, he had made a successful tour of the UK, but when he returned in January 1965 his behavior, perhaps influenced by the injustice of his prison experience, was erratic and moody, and his touring style of using unrehearsed local backing bands and a strict nonnegotiable contract was earning him a reputation as a difficult and unexciting performer. He also played at large events in North America, such as the Schaefer Music Festival, in New York City's Central Park in July 1969, and the Toronto Rock and Roll Revival festival in October.

Berry's touring style, traveling the "oldies" circuit in the 1970s (often being paid in cash by local promoters) added ammunition to the Internal Revenue Service's accusations that Berry had evaded paying income taxes. Facing Criminal sanction for the third time, Berry pleaded guilty to tax evasion and was sentenced to four months in prison and 1,000 hours of community service—performing benefit concerts—in 1979.

Berry announced on his 90th birthday that his first new studio album since Rock It in 1979, entitled Chuck, would be released in 2017. His first new record in 38 years, it includes his children, Charles Berry Jr. and Ingrid, on guitar and harmonica, with songs "covering the spectrum from hard-driving rockers to soulful thought-provoking time capsules of a life's work" and dedicated to his beloved wife of 68 years, Toddy.

In the late 1980s, Berry bought the Southern Air, a restaurant in Wentzville, Missouri. In 1990 he was sued by several women who claimed that he had installed a video camera in the bathroom. Berry claimed that he had had the camera installed to catch a worker who was suspected of stealing from the restaurant. Though his guilt was never proved in court, Berry opted for a class action settlement. (One of his biographers, Bruce Pegg, estimated that, with 59 women, it cost Berry over $1.2 million plus legal fees.). His lawyers said he had been the victim of a conspiracy to profit from his wealth. During this time Berry began using Wayne T. Schoeneberg as his legal counsel. Reportedly, a police raid on his house found intimate videotapes of women, one of whom being apparently a minor. Also found in the raid were 62 grams of marijuana. Felony drug and child-abuse charges were filed. As the child-abuse charges were dropped, Berry agreed to plead guilty to misdemeanor possession of marijuana. He was given a six-month suspended jail sentence and two years' unsupervised probation and was ordered to donate $5,000 to a local hospital.

Berry was among the first Musicians to be inducted into the Rock and Roll Hall of Fame on its opening in 1986; he was cited for having "laid the groundwork for not only a rock and roll sound but a rock and roll stance." Berry is included in several of Rolling Stone magazine's "greatest of all time" lists; he was ranked fifth on its 2004 and 2011 list of the 100 Greatest Artists of All Time. The Rock and Roll Hall of Fame's 500 Songs That Shaped Rock and Roll includes three of Berry's: "Johnny B. Goode", "Maybellene", and "Rock and Roll Music". Berry's "Johnny B. Goode" is the only rock-and-roll song included on the Voyager Golden Record.

Berry lived in Ladue, Missouri, approximately 10 miles (16 km) west of St. Louis. He regularly performed one Wednesday each month at Blueberry Hill, a restaurant and bar located in the Delmar Loop neighborhood of St. Louis, from 1996 to 2014.

In November 2000, Berry faced legal issues when he was sued by his former Pianist Johnnie Johnson, who claimed that he had co-written over 50 songs, including "No Particular Place to Go", "Sweet Little Sixteen" and "Roll Over Beethoven", that credit Berry alone. The case was dismissed when the judge ruled that too much time had passed since the songs were written.

Among the honors Berry received were the Grammy Lifetime Achievement Award in 1984 and the Kennedy Center Honors in 2000. He was ranked seventh on Time magazine's 2009 list of the 10 best electric guitar players of all time. On May 14, 2002, Berry was honored as one of the first BMI Icons at the 50th annual BMI Pop Awards. He was presented the award along with BMI affiliates Bo Diddley and Little Richard. In August 2014, Berry was made a laureate of the Polar Music Prize.

Berry is included in several of Rolling Stone magazine's "Greatest of All Time" lists. In September 2003, the magazine ranked him number 6 in its list of the "100 Greatest Guitarists of All Time". In November his compilation album The Great Twenty-Eight was ranked 21st in Rolling Stone's 500 Greatest Albums of All Time. In March 2004, Berry was ranked fifth on the list of "The Immortals – The 100 Greatest Artists of All Time". In December 2004, six of his songs were included in "Rolling Stone's 500 Greatest Songs of All Time": "Johnny B. Goode" (#7), "Maybellene" (#18), "Roll Over Beethoven" (#97), "Rock and Roll Music" (#128), "Sweet Little Sixteen" (#272) and "Brown Eyed Handsome Man" (#374). In June 2008, his song "Johnny B. Goode" was ranked first in the "100 Greatest Guitar Songs of All Time".

In 2008, Berry toured Europe, with stops in Sweden, Norway, Finland, the United Kingdom, the Netherlands, Ireland, Switzerland, Poland and Spain. In mid-2008, he played at the Virgin Festival in Baltimore, Maryland. During a concert on New Year's Day 2011 in Chicago, Berry, suffering from exhaustion, passed out and had to be helped off stage.

On July 29, 2011, Berry was honored in a dedication of an eight-foot, in-motion Chuck Berry Statue in the Delmar Loop in St. Louis, Missouri, right across the street from Blue Berry Hill. Berry said, "It's glorious--I do appreciate it to the highest, no doubt about it. That sort of honor is seldom given out. But I don't deserve it." (Daniel Durchholz (July 29, 2011). "Chuck Berry Statue Unveiled in St. Louis." "Rolling Stone.")

The Journalist Chuck Klosterman has argued that in 300 years Berry will still be remembered as the rock musician who most closely captured the essence of rock and roll. Time magazine stated, "There was no one like Elvis. But there was 'definitely' no one like Chuck Berry." "Rolling Stone" magazine called him "the father of rock & roll" who "gave the music its sound and its attitude, even as he battled racism - and his own misdeeds - all the way," reporting that Leonard Cohen said, "All of us are footnotes to the words of Chuck Berry." (Mikal Gilmore, "Chuck Berry 1926-2017," "Rolling Stone," pp. 23–24, April 20, 2017) Kevin Strait, curator of the National Museum of African American History and Culture in Washington, DC, said that Berry is "one of the primary sonic Architects of rock and roll." (Kevin Strait (July 6, 2017). "PBS News Hour.")

One of Berry’s attorneys estimated that his estate was worth $50 million, including $17 million in music rights. Berry’s music publishing accounted for $13 million of the estate’s value. The Berry estate owned roughly half of his songwriting credits (mostly from his later career), while BMG Rights Management controlled the other half; most of Berry’s recordings are currently owned by Universal Music Group. In September 2017, Dualtone, the label which released Berry’s final album ‘’Chuck’’, agreed to publish all his compositions in the United States.