Stott was born in Craigieburn, a suburb of Melbourne, Victoria, in 1990. A student of the University of Melbourne, Stott studied Middle Eastern Politics and Immigration Studies, and wrote articles on Middle Eastern affairs. Since 2016, he has been based in Amsterdam, Netherlands.

Stott commenced training as an actor at Screen Actors Australia in 2001, at the age of 11. In 2010, he won a scholarship to the Stella Adler Studio of Acting, A New York City–based acting school founded by American Actress and Writer, Stella Adler. He was named the runner–up to Bella Heathcote for the Heath Ledger Scholarship Award, in 2010.

He was cast in the 2006 Australian adaptation of william Shakespeare's Macbeth, in the role of Fleance. The film, directed by Geoffrey Wright, starred Sam Worthington as Macbeth. For his work in Macbeth, Stott was nominated for the Best Newcomer Award in 2006 by the Australian Film Institute. Also in 2006, Stott appeared in an Australian short film, Teenage Lust. In 2008, Stott appeared in the fourth episode of the eighth season of drama series McLeod's Daughters as Jamie Mitchell, a teenager who has run away from home. Throughout this period in Stott's career, he starred in multiple episodes of the SBS comedy drama Kick in 2007, a guest appearance in the second series of crime drama City Homicide in 2008 and a role in the short film, Leap Year.

His next project was East of Everything, where he landed the regular role of Josh Watkins, a character he portrayed from 2008 to 2009 in the series' two seasons. On his character, Stott drew parallels between himself and Watkins, noting, "He wants to be independent, and that's me all over. At the moment I'm looking to move out. Not because of family problems but because I want my own freedom and that's what Josh is about." His acting was praised by The Age, which heralded Stott's performance as "strong". In 2009, he starred in the Australian film, The Vapour Boys.

Since 2011, Stott has had roles in multiple films, including These Empty Streets (2011), independent comedy horror film Ghost Team One (2013), short films James & Quinn (2013), Grace (2015) and Haven (2015).

While Stott was in Los Angeles in November 2013, he received a brief for a "Gay love story, [set in] Melbourne, 1976" – a role within a film the actor believed "seldom comes along...in Australia’s deeply machismo-oriented society". The film would end up being a film adaption of the 1995 memoir Holding the Man by Australian Writer, actor, and Activist Timothy Conigrave on his life, and centrally of his relationship with his lover of fifteen years, John Caleo. Following auditions and chemistry tests in London, Stott and fellow Australian actor Ryan Corr were selected as the co-leads of Holding the Man, John Caleo and Timothy Conigrave, respectively. Actors Anthony LaPaglia, Guy Pearce and Geoffrey Rush were chosen for supporting roles within the film.

Production on the film was temporarily suspended in 2014 to allow Stott to lose "up to 12 kilograms" for scenes later in the film which show Caleo with AIDS. On portraying Caleo, Stott noted that, "He wasn’t a very complex person with striking, over-arching ambitions. He wasn’t a boisterous or flamboyant character in the way that Tim was. To try and create a character that was really understated, that is who John was...I was always pulling it back. John really comes through in the subtleties, he’s bubbling under the surface constantly."