O'Shaughnessy's most notable stage role to date is that of Salome, for the Gate Theatre in Dublin. Other stage work has included The Shaughraun for the Abbey Theatre in Dublin, which transferred to the West End's Albery Theatre in 2005. In 2006 she appeared in the UK premiere of Blackwater Angel by Jim Nolan at the Finborough Theatre, London. Other work for the Gate Theatre includes Arms and the Man, Oliver, The Importance of Being Earnest, Pride and Prejudice, Blythe Spirit, Present Laughter (which toured in Charleston, South Carolina), See You Next Tuesday, and Cat on a Hot Tin Roof. She played Cate in the Irish debut of Sarah Kane's play Blasted.

Her film roles include Clara in Goldfish Memory, The Halo Effect, and The Stronger. Other theatre work includes playing Hilde Wangle in Lady from the Sea and Petra Stockman in Enemy of the People at the Arcola Theatre, London, and Amy in 'The Night Alive', by Conor Mc Pherson, at The Geffen Playhouse, in Los Angeles, directed by Randall Arney. She stars as Jessica Hyde in the television series Utopia. In 2015, she starred with David Troughton in the comedy romantic horror film Nina Forever, written and directed by the British duo, the Blaine Brothers.