Kapture was born in Yorba Linda, California and raised in Atlanta, Georgia. She began her career starring in films such as Lethal Pursuit (1988), Angel III: The Final Chapter (1988), Liberty & Bash (1989) and The Vagrant (1992), before becoming an international television star. She gained international exposure with her first major television series regular role as Sergeant Rita Lee Lance in the television series Silk Stalkings. Silk Stalkings premiered on CBS and USA but later moved solely to USA Network. "Silk" aired for 8 seasons and became USA network's highest rated original drama series. Her role as Rita, the other half of the two "Sams" for almost 5 years, cemented her respectability as an Actress. Paired with Rob Estes who played Sergeant Chris Lorenzo, Kapture gained global recognition along with her co-star since the show was broadcast in many countries. Sergeant Lance eventually became Lieutenant Lance by the time Kapture left the series in 1995 to have her first daughter. During her time with the series, Kapture also sat in the director's chair.

Kapture returned to television in 1997, starring in two made for USA Network movies. She played lead roles in USA Pictures Originals telemovies, Perfect Crime (based on a true story, also called Hide and Seek: The Joanne Jensen Story), which also starred Jasmine Guy, and His Bodyguard with Robert Guillaume. She returned to series television in a lead role on Baywatch as Alexis Ryker.

From 2002 through 2005, she played the seductive Anita Hodges on the CBS daytime soap opera The Young and the Restless, credited as Mitzi Kapture Donahue. She also starred in the made-for-Lifetime television movie Night of Terror, which premiered March 26, 2007. In her latest film, she played the role of a therapist specializing in autism in the critically acclaimed independent feature God's Ears, released in 2008. In 2010, she guest-starred in an episode of the CBS sitcom Rules of Engagement.