In late 2000 through 2001, she served as the host and ring announcer of the Eric Bischoff led youth-promotion MatRats.

In 2003, she began working for the Hart Family's own promotion, Stampede Wrestling, beginning a long time feud with Belle Lovitz. The pair wrestled each other repeatedly, including a match for the Prairie Wrestling Alliance, where Neidhart teamed with her father, Jim "The Anvil" Neidhart, against Lovitz and Apocalypse. In 2004, however, Neidhart and Lovitz formed a tag team, wrestling several intergender matches against The Meyers Brothers as well as against Anna and Ma Myers.

In mid-2004, she began accepting bookings for a tour in England, and in March 2005, started on a two-month tour of Japan where she used the name Nadia Hart. When she returned to Canada she promptly turned into a villainous character, announcing that "Nasty Nattie" had been born. On June 17, 2005 she defeated Anna Marie, Lovitz, and Ma Myers in a four-way match to become the first Stampede Women's Pacific Champion, though she later vacated the championship. In November 2005, Neidhart damaged her cruciate ligament while wrestling in Japan, and underwent surgery in early 2006. She was sidelined for six months while she recovered.

On October 8, 2006, Neidhart won the SuperGirls Championship from Lisa Moretti at a Big Time Wrestling show held by NWA: Extreme Canadian Championship Wrestling in Newark, California. She held the championship for 19 days, before losing it to Nikki Matthews on October 27 in Surrey, British Columbia. Also in October 2006, she debuted in the all-female promotion, Shimmer Women Athletes. She lost to Sara Del Rey at the tapings of Volume 7, but defeated "The Portuguese Princess" Ariel at the Volume 8 tapings.

On January 5, 2007, Neidhart signed a contract with WWE. Upon signing she was assigned to their developmental territory Deep South Wrestling for the first half of the year, competing against Wrestlers including Krissy Vaine and Angel Williams. She was briefly transferred to Florida Championship Wrestling when it opened in June, and then moved to Ohio Valley Wrestling (OVW), debuting in a dark match at the television tapings on July 18, 2007, where she won a battle royal. She debuted the next week as a member of the Next Generation Hart Foundation, accompanying her cousins Teddy Hart and Harry Smith for tag team matches. In September, she and the rest of the Hart Foundation were moved back to FCW. Initially, Neidhart formed an alliance with Victoria Crawford to take on The Bella Twins (Nikki and Brie Bella), but in 2008, the pair began competing against one another, either in singles matches or in tag team matches with various partners. She continued to make appearances in FCW in late 2008, after debuting on SmackDown, and managed TJ Wilson and DH Smith (Harry Smith) to the FCW Florida Tag Team Championship on October 30.

On the April 11, 2008 episode of SmackDown!, she made her main roster debut as a villain, introducing herself as the daughter of Jim Neidhart under the name Natalya Neidhart, accompanying Victoria to her match against Michelle McCool. In the April 4, episode of SmackDown! she came out of the crowd to aid Victoria in an attack against McCool and Cherry, forming an alliance with her in the process. Three weeks later on SmackDown! she made her televised in-ring debut, under the shortened ring name Natalya, defeating Cherry with her uncle's submission move the "sharpshooter". On the June 6 episode of SmackDown!, SmackDown General Manager Vickie Guerrero announced the creation of the Divas Championship, and that same night the Divas competed in a Golden Dreams match to qualify for the championship match at The Great American Bash. Natalya won, but lost the match to Michelle McCool. At the Backlash pay–per–view on April 27 Natalya teamed up with Victoria, Beth Phoenix, Jillian Hall, Layla and Melina to defeat Mickie James, Maria Kanellis, Ashley, Michelle McCool, Cherry and Kelly Kelly in a 12-Diva tag team match. On the May 2 episode of SmackDown!, Victoria and Natalya defeated McCool and Cherry.

On June 29, 2009, Natalya was traded back to SmackDown along with the other members of The Hart Dynasty. Natalya's first match back for the brand was in a six-person mixed tag team match on the July 17 episode, when The Hart Dynasty defeated Cryme Tyme (JTG and Shad Gaspard) and Eve Torres. Natalya, Kidd, and Smith went on to feud with Torres and Cryme Tyme, with Natalya also facing Torres in singles matches and tag team matches involving other Divas. Natalya was on the winning team when the SmackDown Divas defeated the Raw Divas at the Bragging Rights pay-per-view in October. On December 4, Natalya lost in a triple threat match to determine to number one contender for the WWE Women's Championship after she was pinned by Mickie James. The match also involved Beth Phoenix.

Natalya has appeared in nine WWE video games. She made her in-game debut at WWE SmackDown vs. Raw 2010 and appears in WWE SmackDown vs. Raw 2011, WWE 12, WWE 13 (DLC), WWE 2K14, WWE 2K15, WWE 2K16, WWE 2K17 and WWE 2K18.

On the August 1, 2011, episode of Raw, she competed in a battle royal to determinate the number one contender to Kelly Kelly's Divas Championship at SummerSlam, which was won by Beth Phoenix. Post-match, she turned villainous by attacking Kelly, and stating "[her] days as the perky, cute, blonde little bimbo were officially over", four days later on SmackDown, Natalya defeated AJ before turning on her protégé, attacking her and declaring war on the "perky little princesses" that comprised the rest of the Divas division. As part of the storyline, she aligned herself with Phoenix, and the pair dubbed themselves "The Divas of Doom", defeating The Chickbusters the following week. Throughout September, The Divas of Doom feuded with The Chickbusters on SmackDown, while feuding with Kelly Kelly and Eve Torres on Raw.

Beginning in April, while on NXT Redemption, Natalya became allied with former Hart Dynasty partner Tyson Kidd again, and started feuding with former protégé Kaitlyn when she felt Kaitlyn was paying Kidd too much attention. The pair faced off in both singles and tag team competition throughout mid-2012, including being on opposite sides of a six-Diva tag team match at Money in the Bank, which Natalya's team lost. Their feud ended on August 31, when Natalya lost to Kaitlyn in a singles match on SmackDown. In November, Natalya began an on-screen relationship with The Great Khali. The following month, Natalya saved Hornswoggle from an attack by Rosa Mendes, thus turning face in the process. This led to a feud with Natalya, Khali, and Hornswoggle facing off against Mendes and her allies, Primo and Epico in mixed tag team matches in early 2013. On the March 27 episode of NXT, Natalya lost a Divas Championship match to champion Kaitlyn.

Neidhart has known TJ Wilson since she was 12 years old, and they began dating in November 2001. The pair married on June 26, 2013. Their wedding was featured on the first season of Total Divas. She also owns a home in Calgary

On April 6, 2014, Natalya competed at WrestleMania XXX in the 14–Diva "Vickie Guerrero Invitational match" for the WWE Divas Championship, which was won by the defending champion AJ Lee. In May, Natalya was entered into a tournament to determine the new NXT Women's Champion. She defeated Layla and Sasha Banks en route to the final at NXT TakeOver, in which she lost to Charlotte. Throughout September, Natalya started acting as the on-screen mentor for Rosa Mendes, starting a feud against "The Slayers" (Summer Rae and Layla). They faced off against them during the Main Event and Smackdown shows, losing both tag matches due to miscommunication as a team. Additionally they also faced off against AJ Lee and Paige on the September 8, episode of Raw, once again in a losing effort. On the September 29, episode of Raw, Natalya and Tyson Kidd accompanied Mendes to her match against Layla, in which she was defeated, followed this her association with Mendes quietly disbanded. On November 23, Natalya took part of Alicia Fox's team at Survivor Series, which was victorious in a clean sweep.

After a three-month absence from television due to Tyson Kidd's spinal injury, Natalya made her return on the September 21, 2015 episode of Raw, as a face, confronting the villainous Paige over her actions during Charlotte's WWE Divas Championship celebration. Later that evening, she was defeated by Naomi in a singles match. After multiple confrontations between the two, Natalya would defeat Paige on the October 5 episode of Raw. Three days later, on Smackdown, Natalya teamed with Charlotte and Becky Lynch and the three defeated Team Bella (Alicia Fox, Brie Bella, and Nikki Bella). On the October 15 episode of Smackdown, while Paige saved Charlotte and Becky from an attack by Team Bella, Natalya, after a backstage altercation with Paige, would be attacked. In the end of October, Natalya started a feud with Team B.A.D. (Naomi, Tamina and Sasha Banks), after the three distracted her during her match with Paige, and attacked her, but Natalya went on to gain revenge over the team by defeating Naomi and Tamina in two singles matches before the feud quietly ended.

On October 1, 2016, Neidhart had her two front teeth knocked out during a house show match in Las Vegas.

On the October 25 episode of SmackDown, Natalya competed against Bella in a losing effort to be the captain of the SmackDown women's team at the Survivor Series pay-per-view. At Survivor Series, she replaced Bella in the traditional elimination tag team match against Team Raw after Bella was mysteriously attacked backstage, where Natalya's team was defeated. At the TLC pay-per-view, Carmella revealed that Natalya was Bella's attacker at Survivor Series, which Natalya later denied. After weeks of denying these accusations, on the December 20 episode of SmackDown, Natalya attacked Carmella and admitted to being Bella's attacker, revealing her pent-up jealousy of Bella's success over the years. Natalya and Bella were set to compete in a match on the January 10, 2017, episode of SmackDown, but the match never officially started due a brawl between the two, where referees came in to break them up. At the Royal Rumble pay-per-view on January 29, Natalya competed in a six-woman tag team match alongside Bliss and Mickie James against Bella, Naomi, and Lynch, where her team was defeated after Naomi pinned Bliss. On February 12 at the Elimination Chamber pay-per-view, Natalya faced Bella in singles competition, which ended in a double count-out. The feud culminated in a Falls Count Anywhere match on the February 21 episode of SmackDown, where Natalya defeated Bella after an interference from Maryse. On June 18 at Money in the Bank, Natalya competed in the inaugural women's Money in the Bank ladder match, which was won by Carmella following an interference by James Ellsworth.