In addition to her well-known role in The Gods Must Be Crazy, Prinsloo has had major roles in Target of an Assassin (1979), Quest for Love (1988), Die Prince van Pretoria (1992), Soweto Green (1995), 'n Paw Paw fir My Darling (2015), Twee Grade van Moord (2016).

In 1985, Prinsloo and fellow actor John Kani caused a South African audience walk-out when performing the play Miss Julie by August Strindberg. In the title role, Prinsloo played a white woman seducing a black man. The play marked the first on-stage occurrence of a black man kissing a white woman under the Apartheid regime.

The actors later performed the play at the Edinburgh Festival. In 1986, Heaney directed a TV movie of the play for Swedish and Finnish television, which starred both Prinsloo and Kani.

Prinsloo returned to the Edinburgh Festival in 2012 with The Sewing Machine, an English-language translation of the Afrikaans-language play, Die Naaimasjien. In the 2000s, Prinsloo appeared on the South African stage in productions of Oskar en die Pienk Tannie; Janneman; Nag, Ma (Night Mother); and Liefde, Anna.

In 2013, Prinsloo received the ACT Lifetime Achievement Award for Theatre. The awards are offered annually by the Arts and Culture Trust (ACT) of South Africa in order to "honour arts professionals whose extraordinary careers have had a profound and lasting impact on arts, culture and heritage, and whose lifetime achievements have contributed significantly to the enrichment of cultural life in South Africa".

In 2014, Prinsloo was the recipient of a national order by the South African government, the Order of Ikhamanga in silver, for those whose outstanding work has benefited South Africa. National orders are bestowed on South Africa's Freedom Day. The orders celebrate human achievement in various fields, honouring men and women who have left definitive footprints in both the struggle for liberation and in building a better South Africa.

In October 2015 Prinsloo will portray South African Journalist, Jani Allan in Jani at the Aardklop festival.

“I was a ballet Dancer from a very early age and I remember that I occasionally ushered at the Breytenbach Theatre in Pretoria when I was in high school. That’s the first time I came into contact with professional stage acting, but I never thought I’d be an Actress . . . . I think I was far too shy in those days to ever think I could be an Actress. So it wasn’t like I had this great big burning passion to become an Actress, not at all. I preferred an academic life, in a way. When I went to university, I thought that was going to be what I was going to do”.